Molecular characterization of alterations in the intestinal microbiota of patients with grade 3 hypertension.

ABSTRACT: Hypertension has become a major risk factor for many diseases, including cardiovascular, cerebrovascular and kidney disorders. It has been reported that the composition of human gut microbiota is changed during the progression of cardiovascular and kidney diseases. The current study aimed to qualitatively and quantitatively compare the composition of gut microbiota between patients with hypertension and healthy controls. Fecal samples were collected from 50 patients diagnosed with grade 3 hypertension and 30 healthy controls. Touchdown PCR‑denaturing gradient gel electrophoresis with primers specifically targeting the V3 region of 16S ribosomal RNA, and quantitative PCR, were performed to characterize all the samples. High‑throughput sequencing of the V3‑V4 regions was performed on 30 rand

SUBMITTER: Mushtaq N 

PROVIDER: S-EPMC6605625 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature