DDGun: an untrained method for the prediction of protein stability changes upon single and multiple point variations.

ABSTRACT:

Background

Predicting the effect of single point variations on protein stability constitutes a crucial step toward understanding the relationship between protein structure and function. To this end, several methods have been developed to predict changes in the Gibbs free energy of unfolding (∆∆G) between wild type and variant proteins, using sequence and structure information. Most of the available methods however do not exhibit the anti-symmetric prediction property, which guarantees that the predicted ∆∆G value for a variation is the exact opposite of that predicted for the reverse variation, i.e., ∆∆G(A → B) = -∆∆G(B → A), where A and B are amino acids.

Results

Here we introduce simple anti-symmetric features, based on evolutionary information, which are combined to defin

SUBMITTER: Montanucci L 

PROVIDER: S-EPMC6606456 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature