Generation and Validation of miR-100 Hepatocyte-Specific Knock-Out Mice.

ABSTRACT: Background: Inactivation of microRNA-100 (miR-100) is involved in hepatocellular carcinoma (HCC) and miR-100 behaves as a tumor suppressor. To understand miR-100 function in HCC genesis and development in vivo, we developed hepatocyte-specific miR-100 deficient mice. Methods: Mice homozygous for floxed miR-100 allele that carried the Alb-Cre transgene (miR-100^flox/floxAlb -Cre⁺) were developed by mating miR-100^flox/flox mice with Alb-Cre^+/+mice. The mice tails DNA were genotyped using the primers for LoxP sites and Cre recombinase, respectively. The specific deletion of miR-100 in the livers was verified by quantitative Real-time PCR (qRT-PCR). HE-staining was performed for histology analysis. Liver function was assessed by t

SUBMITTER: Yang D 

PROVIDER: S-EPMC6606737 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature