The Stringent Response Inhibits DNA Replication Initiation in E. coli by Modulating Supercoiling of oriC.

ABSTRACT: The stringent response enables bacteria to respond to a variety of environmental stresses, especially various forms of nutrient limitation. During the stringent response, the cell produces large quantities of the nucleotide alarmone ppGpp, which modulates many aspects of cell physiology, including reprogramming transcription, blocking protein translation, and inhibiting new rounds of DNA replication. The mechanism by which ppGpp inhibits DNA replication initiation in Escherichia coli remains unclear. Prior work suggested that ppGpp blocks new rounds of replication by inhibiting transcription of the essential initiation factor dnaA, but we found that replication is still inhibited by ppGpp in cells ectopically producing DnaA. Instead, we provide evidence that a global reductio

SUBMITTER: Kraemer JA 

PROVIDER: S-EPMC6606810 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature