Differentiation and identification of ginsenoside structural isomers by two-dimensional mass spectrometry combined with statistical analysis.

ABSTRACT:

Background

In the current phytochemical research on ginseng, the differentiation and structural identification of ginsenosides isomers remain challenging. In this paper, a two-dimensional mass spectrometry (2D-MS) method was developed and combined with statistical analysis for the direct differentiation, identification, and relative quantification of protopanaxadiol (PPD)-type ginsenoside isomers.

Methods

Collision-induced dissociation was performed at successive collision energy values to produce distinct profiles of the intensity fraction (IF) and ratio of intensity (RI) of the fragment ions. To amplify the differences in tandem mass spectra between isomers, IF and RI were plotted against collision energy. The resulting data distributions were then used to obtain the param

SUBMITTER: Xiu Y 

PROVIDER: S-EPMC6606828 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature