Time Series Genome-Centric Analysis Unveils Bacterial Response to Operational Disturbance in Activated Sludge.

ABSTRACT: Understanding ecosystem response to disturbances and identifying the most critical traits for the maintenance of ecosystem functioning are important goals for microbial community ecology. In this study, we used 16S rRNA amplicon sequencing and metagenomics to investigate the assembly of bacterial populations in a full-scale municipal activated sludge wastewater treatment plant over a period of 3 years, including a 9-month period of disturbance characterized by short-term plant shutdowns. Following the reconstruction of 173 metagenome-assembled genomes, we assessed the functional potential, the number of rRNA gene operons, and the in situ growth rate of microorganisms present throughout the time series. Operational disturbances caused a significant decrease in bacteria with a single

SUBMITTER: Perez MV 

PROVIDER: S-EPMC6606829 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature