A targeted proteomics approach reveals a serum protein signature as diagnostic biomarker for resectable gastric cancer.

ABSTRACT:

Background

Gastric cancer (GC) is the third leading cause of cancer death. Early detection is a key factor to reduce its mortality.

Methods

We retrospectively collected pre- and postoperative serum samples as well as tumour tissues and adjacent normal tissues from 100 GC patients. Serum samples from non-cancerous patients were served as controls (n = 50). A high-throughput protein detection technology, multiplex proximity extension assays (PEA), was applied to measure levels of over 300 proteins. Alteration of each protein was analysed by univariate analysis. Elastic-net logistic regression was performed to select serum proteins into the diagnostic model.

Findings

We identified 19 serum proteins (CEACAM5, CA9, MSLN, CCL20, SCF, TGF-alpha, MMP-1, MMP-10, IGF-1, CDCP1,

SUBMITTER: Shen Q 

PROVIDER: S-EPMC6606959 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature