Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors.

ABSTRACT:

Purpose

The early transcriptional events that occur in newly generated cone photoreceptors are not well described. Knowledge of these events is critical to provide benchmarks for in vitro-derived cone photoreceptors and to understand the process of cone and rod photoreceptor diversification. We sought to identify genes with differential gene expression in embryonic mouse cone photoreceptors.

Methods

The specificity of expression of the LHX4 transcription factor in developing cone photoreceptors was examined using immunofluorescence visualization in both mouse and chicken retinas. A LHX4 transgenic reporter line with high specificity for developing mouse cone photoreceptors was identified and used to purify early-stage cone photoreceptors for profiling by single-cell RNA sequ

SUBMITTER: Buenaventura DF 

PROVIDER: S-EPMC6607928 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature