Environmental factors influence the Haloferax volcanii S-layer protein structure.

ABSTRACT: S-layers commonly cover archaeal cell envelopes and are composed of proteins that self-assemble into a paracrystalline surface structure. Despite their detection in almost all archaea, there are few reports investigating the structural properties of these proteins, with no reports exploring this topic for halophilic S-layers. The objective of the present study was to investigate the secondary and tertiary organization of the Haloferax volcanii S-layer protein. Such investigations were performed using circular dichroism, fluorescence spectroscopy, dynamic light scattering and transmission electron microscopy. The protein secondary structure is centered on β-sheets and is affected by environmental pH, with higher disorder in more alkaline conditions. The pH can also affect the protein's tert

SUBMITTER: Rodrigues-Oliveira T 

PROVIDER: S-EPMC6607943 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature