Improved Monitoring of Low-Level Transcription in Escherichia coli by a β-Galactosidase α-Complementation System.

ABSTRACT: Genetically encoded reporter proteins are important and widely used tools for the identification and capture of a promoter, tracking the dynamic behavior of transcription, and the quantification of promoter activity. The sensitivity of the reporter gene is a critical factor for an ideal reporter system because weak transcriptional signal has usually failed to be detected using classical reporters. In this study, we present a novel reporter system for improved monitoring of transcription in E. coli based on β-galactosidase α-complementation. In this reporter system, the β-galactosidase activity resulting from the assembly of a reporter lacZα and an existing α-acceptor in advance serves as a measure of transcriptional activity in vivo. To validate the potential of the lacZα-der

SUBMITTER: Guo Y 

PROVIDER: S-EPMC6607957 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature