Downregulation of a CYP74 Rubber Particle Protein Increases Natural Rubber Production in Parthenium argentatum.

ABSTRACT: We report functional genomics studies of a CYP74 rubber particle protein from Parthenium argentatum, commonly called guayule. Previously identified as an allene oxide synthase (AOS), this CYP74 constitutes the most abundant protein found in guayule rubber particles. Transgenic guayule lines with AOS gene expression down-regulated by RNAi (AOSi) exhibited strong phenotypes that included agricultural traits conducive to enhancing rubber yield. AOSi lines had higher leaf and stem biomass, thicker stembark tissues, increased stem branching and improved net photosynthetic rate. Importantly, the rubber content was significantly increased in AOSi lines compared to the wild-type (WT), vector control and AOS overexpressing (AOSoe) lines, when grown i

SUBMITTER: Placido DF 

PROVIDER: S-EPMC6607968 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature