Role of dendritic cell maturation factors produced by human invariant NKT cells in immune tolerance.

ABSTRACT: In this study, we used the culture supernatant of iNKT cells to identify human myeloid DC maturation factors produced by human CD4⁺ iNKT cells. S100A8 had a strong maturation effect. Notably, the recombinant S100A8 protein displayed properties of DC maturation functioning, and the induction of DC differentiation by both the purified and the recombinant protein were blocked by anti-S100A8 and anti-TLR-4 mAbs. DC differentiation induced by anti-major histocompatibility complex class II/CD1d Ab, S100A8, or both was qualitatively indistinguishable from that induced by the coculture of DCs and iNKT cells or via culture supplementation with supernatants from activated CD4⁺ iNKT cells. S100A8 also induced CD4⁺/CD25⁺/Foxp3⁺ T_reg cel

SUBMITTER: Lee HW 

PROVIDER: S-EPMC6608005 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature