Clusterin synergizes with IL-2 for the expansion and IFN-γ production of natural killer cells.

ABSTRACT: CLU is a secreted, multifunctional protein implicated in several immunologic and pathologic conditions. As the level of serum CLU was shown to be elevated during inflammatory responses, we questioned if CLU might interact with circulating lymphocytes leading to functional consequences. To assess this possibility directly, mouse splenocytes and purified NK cells were cultured with varying dose of CLU, and its effect on cell proliferation was examined. Our data showed that CLU up-regulated DNA synthesis and expansion of NK cells significantly in response to a suboptimal, but not maximal, dose of IL-2, and CLU alone did not exhibit such effects. This CLU-mediated synergy required the co-presence of CLU at the onset of IL-2 stimulation and needed a continuous presence during the rest of the cu

SUBMITTER: Sonn CH 

PROVIDER: S-EPMC6608014 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature