Comparative exploration of multidimensional flow cytometry software: a model approach evaluating T cell polyfunctional behavior.

ABSTRACT: Advancement in flow cytometry reagents and instrumentation has allowed for simultaneous analysis of large numbers of lineage/functional immune cell markers. Highly complex datasets generated by polychromatic flow cytometry require proper analytical software to answer investigators' questions. A problem among many investigators and flow cytometry Shared Resource Laboratories (SRLs), including our own, is a lack of access to a flow cytometry-knowledgeable bioinformatics team, making it difficult to learn and choose appropriate analysis tool(s). Here, we comparatively assess various multidimensional flow cytometry software packages for their ability to answer a specific biologic question and provide graphical representation output suitable for publication, as well as their ease of use and cos

SUBMITTER: Spear TT 

PROVIDER: S-EPMC6608077 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature