On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies.

ABSTRACT: Efficient and reliable sample delivery has remained one of the bottlenecks for serial crystallography experiments. Compared with other methods, fixed-target sample delivery offers the advantage of significantly reduced sample consumption and shorter data collection times owing to higher hit rates. Here, a new method of on-chip crystallization is reported which allows the efficient and reproducible growth of large numbers of protein crystals directly on micro-patterned silicon chips for in-situ serial crystallography experiments. Crystals are grown by sitting-drop vapor diffusion and previously established crystallization conditions can be directly applied. By reducing the number of crystal-handling steps, the method is particularly well suited for sensitive crystal systems. Excessiv

SUBMITTER: Lieske J 

PROVIDER: S-EPMC6608620 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature