Etiology of Central Nervous System Infections in a Rural Area of Nepal Using Molecular Approaches.

ABSTRACT: The etiology of infections of the central nervous system (CNS) in Nepal often remains unrecognized because of underdeveloped laboratory facilities. The aim of this study was to investigate the etiology of CNS infections in a rural area of Nepal using molecular methods. From November 2014 to February 2016, cerebrospinal fluid (CSF) was collected from 176 consecutive patients presenting at United Mission Hospital in Tansen, Nepal, with symptoms of possible CNS infection. After the CSF samples were stored and transported frozen, polymerase chain reaction (PCR) was performed in Sweden, targeting a total of 26 pathogens using the FilmArray^® ME panel (BioFire, bioMerieux, Salt Lake City, UT), the MeningoFinder^® 2SMART (PathoFinder, Maastricht, The Netherlands), and an in-ho

SUBMITTER: Sall O 

PROVIDER: S-EPMC6609203 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature