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Imaging neuropeptide release at synapses with a genetically engineered reporter.

ABSTRACT: Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.

SUBMITTER: Ding K

PROVIDER: S-EPMC6609332 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Imaging neuropeptide release at synapses with a genetically engineered reporter.

Ding Keke K Han Yifu Y Seid Taylor W TW Buser Christopher C Karigo Tomomi T Zhang Shishuo S Dickman Dion K DK Anderson David J DJ

eLife 20190626

Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the <i>Drosophila</i> larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimul ...[more]

PMID: 31241464

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