Contribution of potassium channels to action potential repolarization of human embryonic stem cell-derived cardiomyocytes.

ABSTRACT:

Background and purpose

The electrophysiological properties of human pluripotent stem cell-derived cardiomyocytes (CMs) have not yet been characterized in a syncytial context. This study systematically characterized the contributions of different repolarizing potassium currents in human embryonic stem cell-derived CMs (hESC-CMs) during long-term culture as cell monolayers.

Experimental approach

The H9 hESC line was differentiated to CMs and plated to form confluent cell monolayers. Optical mapping was used to record the action potentials (APs) and conduction velocity (CV) during electrophysiological and pharmacological experiments. RT-PCR and Western blot were used to detect the presence and expression levels of ion channel subunits.

Key results

Long-term culture of hE

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6609543 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature