Blockade of the forward Na⁺ /Ca²⁺ exchanger suppresses the growth of glioblastoma cells through Ca²⁺ -mediated cell death.

ABSTRACT:

Background and purpose

The Na⁺ /Ca²⁺ exchanger (NCX) working in either forward or reverse mode participates in maintaining intracellular Ca²⁺ ([Ca²⁺ ]_i ) homeostasis, which is essential for determining cell fate. Previously, numerous blockers targeting reverse or forward NCX have been developed and studied in ischaemic tissue injury but barely examined in glioblastoma for the purpose of anti-tumour therapy. We assessed the effect of NCX blockers on glioblastoma growth and whether NCX can become a therapeutic target.

Experimental approach

Patch-clamp recording, Ca²⁺ imaging, flow cytometry, and Western blot were used to study the effects of specific and non-specific NCX blockers on cultured glioblastoma cells.

SUBMITTER: Hu HJ 

PROVIDER: S-EPMC6609550 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature