Fractionated mitochondrial magnetic separation for isolation of synaptic mitochondria from brain tissue.

ABSTRACT: While mitochondria maintain essential cellular functions, such as energy production, calcium homeostasis, and regulating programmed cellular death, they also play a major role in pathophysiology of many neurological disorders. Furthermore, several neurodegenerative diseases are closely linked with synaptic damage and synaptic mitochondrial dysfunction. Unfortunately, the ability to assess mitochondrial dysfunction and the efficacy of mitochondrial-targeted therapies in experimental models of neurodegenerative disease and CNS injury is limited by current mitochondrial isolation techniques. Density gradient ultracentrifugation (UC) is currently the only technique that can separate synaptic and non-synaptic mitochondrial sub-populations, though small brain regions cannot be assayed due to low

SUBMITTER: Hubbard WB 

PROVIDER: S-EPMC6609636 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature