High-throughput analysis of the human thymic V?1+ T cell receptor repertoire.

ABSTRACT: ?? T cells are a relatively rare subset of lymphocytes in the human peripheral blood, but they play important roles at the interface between the innate and the adaptive immune systems. The ?? T cell lineage is characterized by a signature ?? T cell receptor (??TCR) that displays extensive sequence variability originated by DNA rearrangement of the corresponding V(D)J loci. Human ?? T cells comprise V?9V?2 T cells, the major subset in the peripheral blood; and V?1+ T cells, the predominant subpopulation in the post-natal thymus and in peripheral tissues. While less studied, V?1+ T cells recently gathered significant attention due to their anti-cancer and anti-viral activities. In this study we applied next-generation sequencing (NGS) to analyse the ??TCR repertoire of highly (FACS-)purified V?1+ T cells from human thymic biopsies. Our analysis reveals unsuspected aspects of thymically rearranged and expressed (at the mRNA level) TRG and TRD genes, thus constituting a data resource that qualifies previous conclusions on the TCR repertoire of ?? T cells developing in the human thymus.

SUBMITTER: Di Lorenzo B 

PROVIDER: S-EPMC6609709 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature