Regulation of outer-membrane proteins (OMPs) A and F, during hlyF-induced outer-membrane vesicle (OMV) biosynthesis.

ABSTRACT:

Background

Gram-negative bacteria actively secrete outer membrane vesicles into the surrounding environment and these vesicles have been shown to play various physiological and protective roles such as carrying antibiotic-degrading enzymes and acting as decoys against host defences, therefore promoting the pathogenesis of the bacterium. It has been shown that avian pathogenic Escherichia coli species can increase vesicle biosynthesis through the acquisition of the hlyF gene but the effect this has on the cell by scavenging outer-membrane associated proteins (OmpA, OmpF) into the vesicles during vesicle release have not yet been investigated.

Results

Relative quantitative real-time PCR data obtained from hlyF expressing and non-expressing cells showed tha

SUBMITTER: van der Westhuizen WA 

PROVIDER: S-EPMC6610587 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature