Modulation of chimeric antigen receptor surface expression by a small molecule switch.

ABSTRACT: BACKGROUND:Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity. Chimeric antigen receptor (CAR) T-cells are designed to detect and kill tumor cells that present a specific, predefined antigen. The rapid expansion of targeted antigen beyond CD19, has highlighted new challenges, such as autoactivation and T-cell fratricide, that could impact the capacity to manufacture engineered CAR T-cells. Therefore, the development of strategies to control CAR expression at the surface of T-cells and their functions is under intense investigations. RESULTS:Here, we report the development and evaluation of an off-switch directly embedded within a CAR construct (SWIFF-CAR). The incor

SUBMITTER: Juillerat A 

PROVIDER: S-EPMC6610870 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature