Genome-wide identification and functional analysis of oleosin genes in Brassica napus L.

ABSTRACT:

Background

Rapeseed is the third largest oil seed crop in the world. The seeds of this plant store lipids in oil bodies, and oleosin is the most important structural protein in oil bodies. However, the function of oleosin in oil crops has received little attention.

Results

In the present study, 48 oleosin sequences from the Brassica napus genome were identified and divided into four lineages (T, U, SH, SL). Synteny analysis revealed that most of the oleosin genes were conserved, and all of these genes experienced purifying selection during evolution. Three and four important oleosin genes from Arabidopsis and B. napus, respectively, were cloned and analyzed for function in Arabidopsis. Overexpression of these oleosin genes in Arabidopsis increased the seed oil content slight

SUBMITTER: Chen K 

PROVIDER: S-EPMC6610931 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature