Transcriptome analysis of the fish pathogen Flavobacterium columnare in biofilm suggests calcium role in pathogenesis.

ABSTRACT:

Background

Flavobacterium columnare is the causative agent of columnaris disease that affects cultured freshwater fishes worldwide. F. columnare easily colonizes surfaces by forming biofilm, which helps the pathogen resist antibiotic and disinfectant treatments. Previously, we had shown that increasing concentrations of calcium (Ca²⁺) promoted biofilm formation by F. columnare. The objective of this study was to further characterize the role of Ca²⁺ on biofilm formation and to compare the transcriptome profiles of planktonic and biofilm cells.

Results

RNA-Seq analysis was conducted to identify genes that were differentially expressed between the following states: i) planktonic cells in control medium (P), ii) planktonic cells in calcium-enriched medium

SUBMITTER: Cai W 

PROVIDER: S-EPMC6610971 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature