A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos.

ABSTRACT:

Background

The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence shows that MEK signalling plays a limited role in the segregation of this lineage. Here we explored the role of different signalling pathways during lineage segregation in the bovine embryo using immunofluorescence analysis of NANOG and SOX17 as readouts of epiblast and hypoblast, respectively.

Results

We show that SOX17 starts to be expressed in 16-32-cell stage embryos, whereas NANOG is first detected from 8-cell stage. SOX17 is first co-expressed with NANOG, but these markers become mutually exclusive by the late blastocyst stag

SUBMITTER: Canizo JR 

PROVIDER: S-EPMC6610975 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature