TLR4 participates in the transmission of ethanol-induced neuroinflammation via astrocyte-derived extracellular vesicles.

ABSTRACT:

Background

Current evidence indicates that extracellular vesicles (EVs) participate in intercellular signaling, and in the regulation and amplification of neuroinflammation. We have previously shown that ethanol activates glial cells through Toll-like receptor 4 (TLR4) by triggering neuroinflammation. Here, we evaluate if ethanol and the TLR4 response change the release and inflammatory content of astrocyte-derived EVs, and whether these vesicles are capable of communicating with neurons by spreading neuroinflammation.

Methods

Cortical neurons and astrocytes in culture were used. EVs were isolated from the extracellular medium of the primary culture of the WT and TLR4-KO astrocytes treated with or without ethanol (40 mM) for 24 h. Flow cytometry, nanoparticle tracking analys

SUBMITTER: Ibanez F 

PROVIDER: S-EPMC6610989 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature