Production and purification of mannan oligosaccharide with epithelial tight junction enhancing activity.

ABSTRACT:

Background

Mannanan oligosaccharide (MOS) is well-known as effective supplement food for livestock to increase their nutrients absorption and health status, but the structure and identification of bioactive MOS remain unclear. In this study, MOS production was accomplished, using enzymatic hydrolysis of pretreated coconut meal substrate with recombinant mannanase.

Methods

The mannanase gene was cloned from Bacillus subtilis cAE24, then expressed in BL21. Purified Mannanase exhibit stability over a wide range of pH and temperature from pH 6-8 and 4 °C to 70 °C, respectively. SEM analysis revealed that sonication could change the surface characteristic of copra meal, which gave better MOS yield, compared to untreated substrates. The separation and purification of each M

SUBMITTER: Nopvichai C 

PROVIDER: S-EPMC6611449 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature