A Structural Basis for ¹²⁹ Xe Hyper-CEST Signal in TEM-1 β-Lactamase.

ABSTRACT: Genetically encoded (GE) contrast agents detectable by magnetic resonance imaging (MRI) enable non-invasive visualization of gene expression and cell proliferation at virtually unlimited penetration depths. Using hyperpolarized ¹²⁹ Xe in combination with chemical exchange saturation transfer, an MR contrast approach known as hyper-CEST, enables ultrasensitive protein detection and biomolecular imaging. GE MRI contrast agents developed to date include nanoscale proteinaceous gas vesicles as well as the monomeric bacterial proteins TEM-1 β-lactamase (bla) and maltose binding protein (MBP). To improve understanding of hyper-CEST NMR with proteins, structural and computational studies were performed to further characterize the Xe-bla interaction. X-ray crystallography validated the

SUBMITTER: Roose BW 

PROVIDER: S-EPMC6611679 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature