Comprehensive Metabolomic Analysis of IDH1^R132H Clinical Glioma Samples Reveals Suppression of β-oxidation Due to Carnitine Deficiency.

ABSTRACT: Gliomas with Isocitrate dehydrogenase 1 (IDH1) mutation have alterations in several enzyme activities, resulting in various metabolic changes. The aim of this study was to determine a mechanism for the better prognosis of gliomas with IDH mutation by performing metabolomic analysis. To understand the metabolic state of human gliomas, we analyzed clinical samples obtained from surgical resection of glioma patients (grades II-IV) with or without the IDH1 mutation, and compared the results with U87 glioblastoma cells overexpressing IDH1 or IDH1^R132H. In clinical samples of gliomas with IDH1 mutation, levels of D-2-hydroxyglutarate (D-2HG) were increased significantly compared with gliomas without IDH mutation. Gliomas with IDH mutation also showed decreased intermediates in the tri

SUBMITTER: Miyata S 

PROVIDER: S-EPMC6611790 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature