Proteomic Analysis for the Diagnosis of Fibrinogen Aα-chain Amyloidosis.

ABSTRACT:

Introduction

Hereditary fibrinogen Aα-chain (AFib) amyloidosis is a relatively uncommon renal disease associated with a small number of pathogenic fibrinogen Aα (FibA) variants; wild-type FibA normally does not result in amyloid deposition. Proteomics is now routinely used to identify the amyloid type in clinical samples, and we report here our algorithm for identification of FibA in amyloid.

Methods

Proteomics data from 1001 Congo red-positive patient samples were examined using the Mascot search engine to interrogate the Swiss-Prot database and generate protein identity scores. An algorithm was applied to identify FibA as the amyloid protein based on Mascot scores. FibA variants were identified by appending the known amyloidogenic variant sequences to the Swiss-Prot databa

SUBMITTER: Taylor GW 

PROVIDER: S-EPMC6612008 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature