IL-27 enhances IL-15/IL-18-mediated activation of human natural killer cells.

ABSTRACT:

Background

Natural killer (NK) cells are an emerging new tool for cancer immunotherapy. To develop NK cell therapeutics from peripheral blood mononuclear cells (PBMCs) of healthy donors, substantial expansion of primary NK cells is necessary because of the very low number of these cells in peripheral blood. In this study, we aimed to investigate the effect of various cytokine alone or combinations, in expanded NK cells and to analyze the synergetic effect of cytokine combinations.

Methods

Human NK cells were isolated from healthy donor PBMC. Purified NK cells were stimulated with single cytokines or combinations of IL-2, IL-15, IL-18, and IL-27. The expanded NK cells were characterized by flow cytometry, cytotoxicity assay, calcein AM assay and Western blot.

Results

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SUBMITTER: Choi YH 

PROVIDER: S-EPMC6612093 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature