2HybridTools, a handy software to facilitate clone identification and mutation mapping from yeast two-hybrid screening.

ABSTRACT: Yeast Two-Hybrid (Y2H) and reverse Two-Hybrid (RY2H) are powerful protein-protein interaction screening methods that rely on the interaction of bait and prey proteins fused to DNA binding (DB) and activation domains (AD), respectively. Y2H allows identification of protein interaction partners using screening libraries, while RY2H is used to determine residues critical to a given protein-protein interaction by exploiting site-directed mutagenesis. Currently, both these techniques still rely on sequencing of positive clones using conventional Sanger sequencing. For Y2H, a screen can yield several positives; the identification of such clones is further complicated by the fact that sequencing products usually contain vector sequence. For RY2H, obtaining a complete sequence is required to ident

SUBMITTER: Cauchy P 

PROVIDER: S-EPMC6612259 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature