Towards optimized viral metagenomes for double-stranded and single-stranded DNA viruses from challenging soils.

ABSTRACT: Soils impact global carbon cycling and their resident microbes are critical to their biogeochemical processing and ecosystem outputs. Based on studies in marine systems, viruses infecting soil microbes likely modulate host activities via mortality, horizontal gene transfer, and metabolic control. However, their roles remain largely unexplored due to technical challenges with separating, isolating, and extracting DNA from viruses in soils. Some of these challenges have been overcome by using whole genome amplification methods and while these have allowed insights into the identities of soil viruses and their genomes, their inherit biases have prevented meaningful ecological interpretations. Here we experimentally optimized steps for generating quantitatively-amplified viral metagenomes to b

SUBMITTER: Trubl G 

PROVIDER: S-EPMC6612421 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature