Oligomeric protein interference validates druggability of aspartate interconversion in Plasmodium falciparum.

ABSTRACT: The appearance of multi-drug resistant strains of malaria poses a major challenge to human health and validated drug targets are urgently required. To define a protein's function in vivo and thereby validate it as a drug target, highly specific tools are required that modify protein function with minimal cross-reactivity. While modern genetic approaches often offer the desired level of target specificity, applying these techniques is frequently challenging-particularly in the most dangerous malaria parasite, Plasmodium falciparum. Our hypothesis is that such challenges can be addressed by incorporating mutant proteins within oligomeric protein complexes of the target organism in vivo. In this manuscript, we provide data to support our hypothesis by demonstrating that recombinant expression

SUBMITTER: Batista FA 

PROVIDER: S-EPMC6612543 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature