A collection of barcoded natural isolates of Saccharomyces paradoxus to study microbial evolutionary ecology.

ABSTRACT: While the use of barcoded collections of laboratory microorganisms and the development of barcode-based cell tracking are rapidly developing in genetics and genomics research, tools to track natural populations are still lacking. The yeast Saccharomyces paradoxus is an emergent microbial model in ecology and evolution. More than five allopatric and sympatric lineages have been identified and hundreds of strains have been isolated for this species, allowing to assess the impact of natural diversity on complex traits. We constructed a collection of 550 barcoded and traceable strains of S. paradoxus, including all three North American lineages SpB, SpC, and SpC*. These strains are diploid, many have their genome fully sequenced and are barcoded with a unique 20 bp sequence that allows their i

SUBMITTER: Bleuven C 

PROVIDER: S-EPMC6612553 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature