Effect of Cryopreservation on Autologous Chimeric Antigen Receptor T Cell Characteristics.

ABSTRACT: As clinical applications for chimeric antigen receptor T cell (CART) therapy extend beyond early phase trials, commercial manufacture incorporating cryopreservation steps becomes a logistical necessity. The effect of cryopreservation on CART characteristics is unclear. We retrospectively evaluated the effect of cryopreservation on product release criteria and in vivo characteristics in 158 autologous CART products from 6 single-center clinical trials. Further, from 3 healthy donor manufacturing runs, we prospectively identified differentially expressed cell surface markers and gene signatures among fresh versus cryopreserved CARTs. Within 2 days of culture initiation, cell viability of the starting fraction (peripheral blood mononuclear cells [PBMNCs]) decreased significantly in the cryo-t

SUBMITTER: Panch SR 

PROVIDER: S-EPMC6612799 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature