A statistical simulator scDesign for rational scRNA-seq experimental design.

ABSTRACT:

Motivation

Single-cell RNA sequencing (scRNA-seq) has revolutionized biological sciences by revealing genome-wide gene expression levels within individual cells. However, a critical challenge faced by researchers is how to optimize the choices of sequencing platforms, sequencing depths and cell numbers in designing scRNA-seq experiments, so as to balance the exploration of the depth and breadth of transcriptome information.

Results

Here we present a flexible and robust simulator, scDesign, the first statistical framework for researchers to quantitatively assess practical scRNA-seq experimental design in the context of differential gene expression analysis. In addition to experimental design, scDesign also assists computational method development by generating high-quality sy

SUBMITTER: Li WV 

PROVIDER: S-EPMC6612870 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature