Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation.

ABSTRACT: DNA nanotechnology provides an avenue for the construction of rationally designed artificial assemblages with well-defined and tunable architectures. Shaped to mimic natural membrane-deforming proteins and equipped with membrane anchoring molecules, curved DNA nanostructures can reproduce subcellular membrane remodeling events such as vesicle tubulation in vitro. To systematically analyze how structural stiffness and membrane affinity of DNA nanostructures affect the membrane remodeling outcome, here we build DNA-origami curls with varying thickness and amphipathic peptide density, and have them polymerize into nanosprings on the surface of liposomes. We find that modestly reducing rigidity and maximizing the number of membrane anchors not only promote membrane binding and remodeling but a

SUBMITTER: Grome MW 

PROVIDER: S-EPMC6613048 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature