How the avidity of polymerase binding to the -35/-10 promoter sites affects gene expression.

ABSTRACT: Although the key promoter elements necessary to drive transcription in Escherichia coli have long been understood, we still cannot predict the behavior of arbitrary novel promoters, hampering our ability to characterize the myriad sequenced regulatory architectures as well as to design new synthetic circuits. This work builds upon a beautiful recent experiment by Urtecho et al. [G. Urtecho, et al, Biochemistry, 68, 1539-1551 (2019)] who measured the gene expression of over 10,000 promoters spanning all possible combinations of a small set of regulatory elements. Using these data, we demonstrate that a central claim in energy matrix models of gene expression-that each promoter element contributes independently and additively to gene expression-contradicts experimental m

SUBMITTER: Einav T 

PROVIDER: S-EPMC6613100 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature