Structural knowledge or X-ray damage? A case study on xylose isomerase illustrating both.

ABSTRACT: Xylose isomerase (XI) is an industrially important metalloprotein studied for decades. Its reaction mechanism has been postulated to involve movement of the catalytic metal cofactor to several different conformations. Here, a dose-dependent approach was used to investigate the radiation damage effects on XI and their potential influence on the reaction mechanism interpreted from the X-ray derived structures. Radiation damage is still one of the major challenges for X-ray diffraction experiments and causes both global and site-specific damage. In this study, consecutive high-resolution data sets from a single XI crystal from the same wedge were collected at 100 K and the progression of radiation damage was tracked over increasing dose (0.13-3.88 MGy). The catalytic metal and its surrounding

SUBMITTER: Taberman H 

PROVIDER: S-EPMC6613113 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature