Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function.

ABSTRACT: The spatial regulation of messenger RNA (mRNA) translation is central to cellular functions and relies on numerous complex processes. Biomimetic approaches could bypass these endogenous complex processes, improve our comprehension of the regulation, and allow for controlling local translation regulations and functions. However, the causality between local translation and nascent protein function remains elusive. Here, we developed a nanoparticle (NP)-based strategy to magnetically control mRNA spatial patterns in mammalian cell extracts and investigate how local translation impacts nascent protein localization and function. By monitoring the translation of the magnetically localized mRNAs, we show that mRNA-NP complexes operate as a source for the continuous production of proteins from def

SUBMITTER: Kashida S 

PROVIDER: S-EPMC6613171 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature