Real-time local oxygen measurements for high resolution cellular imaging.

ABSTRACT: Single-cell metabolic investigations are hampered by the absence of flexible tools to measure local partial pressure of O₂ (pO₂) at high spatial-temporal resolution. To this end, we developed an optical sensor capable of measuring local pericellular pO₂ for subcellular resolution measurements with confocal imaging while simultaneously carrying out electrophysiological and/or chemo-mechanical single cell experiments. Here we present the OxySplot optrode, a ratiometric fluorescent O₂-micro-sensor created by adsorbing O₂-sensitive and O₂-insensitive fluorophores onto micro-particles of silica. To protect the OxySplot optrode from the components and reactants of liquid environment without compromising access to O₂, th

SUBMITTER: Boyman L 

PROVIDER: S-EPMC6613206 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature