RNF43 ubiquitinates and degrades phosphorylated E-cadherin by c-Src to facilitate epithelial-mesenchymal transition in lung adenocarcinoma.

ABSTRACT:

Background

In epithelial cells, tyrosine kinases induce tyrosine phosphorylation and ubiquitination of the E-cadherin complex, which is responsible for the epithelial-mesenchymal transition (EMT). However, the precise mechanisms remain unclear.

Methods

Protein antibody microarray analysis and E3 ligase profiling were performed to detect the unique E3 ligase underlying E-cadherin downregulation in lung adenocarcinoma tissues. Gene knockdown was performed using viral shRNA. Immunoblotting, immunofluorescence, immunoprecipitation, and xenograft models in vivo were integratively applied to explore RNF43-induced EMT in lung adenocarcinoma cell lines.

Results

Protein antibody microarray analysis and E3 ligase profiling revealed that the RING finger protein 43 (RNF43) was li

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6613270 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature