Functional Characterization of the ycjQRS Gene Cluster from Escherichia coli: A Novel Pathway for the Transformation of d-Gulosides to d-Glucosides.

ABSTRACT: A combination of bioinformatics, steady-state kinetics, and NMR spectroscopy has revealed the catalytic functions of YcjQ, YcjS, and YcjR from the ycj gene cluster in Escherichia coli K-12. YcjS was determined to be a 3-keto-d-glucoside dehydrogenase with a k_cat = 22 s^-1 and k_cat/ K_m = 2.3 × 10⁴ M^-1 s^-1 for the reduction of methyl α-3-keto-d-glucopyranoside at pH 7.0 with NADH. YcjS also exhibited catalytic activity for the NAD⁺-dependent oxidation of d-glucose, methyl β-d-glucopyranoside, and 1,5-anhydro-d-glucitol. YcjQ was determined to be a 3-keto-d-guloside dehydrogenase with k_cat = 18 s^-1 and k_cat/ K_m = 2.0 × 10³ M^-1 s^-1

SUBMITTER: Mukherjee K 

PROVIDER: S-EPMC6613369 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature