Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics.

ABSTRACT: A technology that simultaneously records membrane potential from multiple neurons in behaving animals will have a transformative effect on neuroscience research^1,2. Genetically encoded voltage indicators are a promising tool for these purposes; however, these have so far been limited to single-cell recordings with a marginal signal-to-noise ratio in vivo^3-5. Here we developed improved near-infrared voltage indicators, high-speed microscopes and targeted gene expression schemes that enabled simultaneous in vivo recordings of supra- and subthreshold voltage dynamics in multiple neurons in the hippocampus of behaving mice. The reporters revealed subcellular details of back-propagating action potentials and correlations in subthreshold voltage between multiple cells. In c

SUBMITTER: Adam Y 

PROVIDER: S-EPMC6613938 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature