Impairment of starch biosynthesis results in elevated oxidative stress and autophagy activity in Chlamydomonas reinhardtii.

ABSTRACT: Autophagy is a self-degradation system wherein cellular materials are recycled. Although autophagy has been extensively studied in yeast and mammalian systems, integrated stress responses in microalgae remain poorly understood. Accordingly, we carried out a comparative study on the oxidative stress responses of Chlamydomonas reinhardtii wild-type and a starchless (sta6) mutant previously shown to accumulate high lipid content under adverse conditions. To our surprise, the sta6 mutant exhibited significantly higher levels of lipid peroxidation in the same growth conditions compared to controls. The sta6 mutant was more sensitive to oxidative stress induced by H₂O₂, whereas the wild-type was relatively more resistant. In addition, significantly up-regulated autophagy-re

SUBMITTER: Tran QG 

PROVIDER: S-EPMC6614365 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature