Phylogenetic and crystallographic analysis of Nostoc phycocyanin having blue-shifted spectral properties.

ABSTRACT: The distinct sequence feature and spectral blue-shift (~10 nm) of phycocyanin, isolated from Nostoc sp. R76DM (N-PC), were investigated by phylogenetic and crystallographic analyses. Twelve conserved substitutions in N-PC sequence were found distributed unequally among α- and β-subunit (3 in α- and 9 in β-subunit). The phylogenetic analysis suggested that molecular evolution of α- and β-subunit of Nostoc-phycocyanin is faster than evolution of Nostoc-species. The divergence events seem to have occurred more frequently in β-subunit, compared to α-subunit (relative divergence, 7.38 for α-subunit and 9.66 for β-subunit). Crystal structure of N-PC was solved at 2.35 Å resolution to reasonable R-factors (R_work/R_Free = 0.199/0.248). Substitutions congregate near interface o

SUBMITTER: Sonani RR 

PROVIDER: S-EPMC6614406 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature