High throughput development of TCR-mimic antibody that targets survivin-2B_80-88/HLA-A*A24 and its application in a bispecific T-cell engager.

ABSTRACT: Intracellular tumor-associated antigens are targeted by antibodies known as T-cell receptor mimic antibodies (TCRm-Abs), which recognize T-cell epitopes with better stabilities and higher affinities than T-cell receptors. However, TCRm-Abs have been proven difficult to produce using conventional techniques. Here, we developed TCRm-Abs that recognize the survivin-2B-derived nonamer peptide, AYACNTSTL (SV2B_80-88), presented on HLA-A*24 (SV2B_80-88/HLA-A*24) from immunized mice by using a fluorescence-activated cell sorting-based antigen-specific plasma cells isolation method combined with a high-throughput single-cell-based immunoglobulin-gene-cloning technology. This approach yielded a remarkable efficiency in generating candidate antibody clones that recognize SV2B

SUBMITTER: Kurosawa N 

PROVIDER: S-EPMC6614450 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature