Monitoring of system conditioning after blank injections in untargeted UPLC-MS metabolomic analysis.

ABSTRACT: Ultra-performance liquid chromatography - mass spectrometry (UPLC-MS) is widely used for untargeted metabolomics in biomedical research. To optimize the quality and precision of UPLC-MS metabolomic analysis, evaluation of blank samples for the elimination of background features is required. Although blanks are usually run either at the beginning or at the end of a sequence of samples, a systematic analysis of their effect of the instrument performance has not been properly documented. Using the analysis of two common bio-fluids (plasma and urine), we describe how the injection of blank samples within a sequence of samples may affect both the chromatographic and MS detection performance depending on several factors, including the sample matrix and the physicochemical properties of the metab

SUBMITTER: Martinez-Sena T 

PROVIDER: S-EPMC6614502 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature